Introduction to QuEChERS
QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) is a recent rapid development of a rapid sample preparation technology for agricultural product testing in the world. It was developed by Professor Anastassiades of the US Department of Agriculture in 2003.
The QuEChERS principle is similar to high performance liquid chromatography (HPLC) and solid phase extraction (SPE). It uses the adsorbent filler to interact with impurities in the matrix to adsorb impurities and achieve impurity removal.
The steps of the QuEChERS method can be summarized as follows:
(1) sample pulverization;
(2) single solvent acetonitrile extraction and separation;
(3) addition of MgSO4 and other salts
(4) Adding an adsorbent such as ethylenediamine-N-propylsilane (PSA) to remove impurities;
(5) The supernatant is subjected to GC-MS and LC-MS.
The Hawach QuEChERS method has the following advantages:
(1) high recovery rate, recovery rate of more than 85% for a large number of polar and volatile pesticides;
(2) high accuracy and accuracy, which can be corrected by internal standard method;
(3) A wide range of analyzable pesticides, including polar and non-polar pesticides, can be used to obtain better recovery rate;
(4) fast analysis, can complete 6 samples in 30 minutes;
(5) The solvent is used in a small amount, the pollution is small, the price is low, and the chloride-containing solvent is not used;
(6) the operation is simple and can be completed without good training and high skill;
(7) the acetonitrile is sealed immediately after being added to the container, so that The chance of contact with the staff is reduced;
(8) few glassware is used in the sample preparation process, and the device is simple.