Hawach QuEChERS Extraction / Purification Tube AOAC Method

HAWACH is a supplier of advanced laboratory equipment, dedicated to providing sales and services of special applications, and advanced laboratory equipment for sample preparation, gas sampling, water, and soil sampling, laboratory and life science filtration and medical-related products. HAWACH’s philosophy is to provide customers with high-performance instruments, high-quality customized solutions, from sales to rapid customization services.

AOAC method
QuEChERS is a sample preparation method proposed by Anastassiades in 2003. It is now widely used in food, environmental water samples, and soil contaminants; Refer to the AOAC Standard (AOAC Official Method 2007.01 Pesticide Residues in Foods by Acetonitrile Extraction and Partitioning with Magnesium Sulfate), and select QuEChERS according to different samples.

HAWACH -QuEChERS AOAC method

Base material Sample volume Extraction solvent Extraction tube Purification tube
General fruits and vegetables

(Removal of polar organic acids, some sugars, and lipids)

15 g 10 ml 1%

Acetonitrile acetate

6 g MgSO4
1.5 g NaOAc
Salt pack
Salt pack + 50 ml centrifuge tube
Salt pack + 50 ml centrifuge tube + homogenous proton
50 mg PSA
150 mg MgSO4
2 ml purification tube
400 mg PSA
1200 mg MgSO4
15 ml purification tube
Fat and waxy fruits and vegetables

(Removal of polar organic acids, certain sugars, most lipids, and sterols)

15 g 10 ml 1%
Acetonitrile acetate
6 g MgSO4
1.5 g NaOAc
Salt pack
Salt pack + 50 ml centrifuge tube
Salt pack + 50 ml centrifuge tube + homogenous proton
50 mg PSA
50 mg C18
150 mg MgSO4
2 ml purification tube400 mg PSA
400 mg C18
1200 mg MgSO4
15 ml purification tube
Pigmented fruits and vegetables

(Removal of polar organic acids, certain sugars, and lipids, as well as carotenoids and chlorophyll)

15 g 10 ml 1%

Acetonitrile acetate

6 g MgSO4
1.5 g NaOAc
Salt pack
Salt pack + 50 ml centrifuge tube
Salt pack + 50 ml centrifuge tube + homogenous proton
50 mg PSA
50 mg GCB
150 mg MgSO4
2 ml purification tube400 mg PSA
400 mg GCB
1200 mg MgSO4
15 ml purification tube
Fruits and vegetables containing fats and pigments

(Removal of polar organic acids, certain sugars, and lipids, as well as carotenoids and chlorophyll)

15 g 10 ml 1%

Acetonitrile acetate

6 g MgSO4
1.5 g NaOAc
Salt pack
Salt pack + 50 ml centrifuge tube
Salt pack + 50 ml centrifuge tube + homogenous proton
50 mg PSA
50 mg C18
50 mg GCB
150 mg MgSO4
2 ml purification tube400 mg PSA
400 mg C18
400 mg GCB
1200 mg MgSO4
15 ml purification tube
Other food methods

(Removal of biological matrix interference, including hydrophobic substances and proteins)

15 g 10 ml 1%

Acetonitrile acetate

6 g MgSO4
1.5 g NaOAc
Salt pack
Salt pack + 50 ml centrifuge tube
Salt pack + 50 ml centrifuge tube + homogenous proton
25 mg C18
150 mg MgSO4
2 ml purification tube150 mg C18
900 mg MgSO4
15 ml purification tube
All food types

(Removal of almost all matrix interferences, including polar organic acids, lipids, sugars, proteins)

15 g 10 ml 1%

Acetonitrile acetate

6 g MgSO4
1.5 g NaOAc
Salt pack
Salt pack + 50 ml centrifuge tube
Salt pack + 50 ml centrifuge tube + homogenous proton
50 mg PSA
50 mg C18
7.5 mg GCB
150 mg MgSO4
2 ml purification tube400 mg PSA
400 mg C18
45 mg GCB
1200 mg MgSO4
15 ml purification tube